Bloodwork Reference Range Lookup
Context-aware lab reference ranges for TRT, HRT, and GLP-1 communities. Compare your results against standard, optimal, and community-validated targets.
Total Testosterone
Total circulating testosterone — the primary androgen. Includes both free and bound fractions. The most commonly ordered testosterone test.
Optimal Timing
Draw at trough — morning, before your next injection. For weekly TRT: day of injection, before injecting.
Reference Ranges
| Context | Range | Source |
|---|---|---|
| Adult Male (20-49) | 249–836 ng/dL | Quest Diagnostics |
| Adult Male (50+) | 193–740 ng/dL | Quest Diagnostics |
| Adult Female | 8–48 ng/dL | Quest Diagnostics |
| TRT Target | 450–750 ng/dL | Endocrine Society (TRT target) |
| TRT Optimal | 700–1100 ng/dL | Community Optimal (TRT) |
| Transfem HRT Target | 10–55 ng/dL | UCSF Transfem |
| Transmasc HRT Target | 300–1000 ng/dL | UCSF Transmasc |
Optimal Bloodwork Timing
For TRT users: draw blood at trough — the morning of your injection, before injecting. This gives the most clinically useful number because it shows your lowest point. If your trough is in range, your peaks will be too. For GLP-1 users: HbA1c doesn't require fasting. For lipid panels: fast for 9-12 hours.
FAQ
Which labs should I get on TRT?+
Essential: Total T, Free T, Estradiol (sensitive), CBC (hematocrit), PSA. Recommended: SHBG, Lipid panel, CMP (liver/kidney). Frequency: every 3-6 months for the first year, then annually.
Should I use the sensitive estradiol assay?+
Yes — always request the sensitive (LC/MS-MS) estradiol assay for males and transmasc patients. The standard immunoassay can be inaccurate at low levels and show falsely elevated readings.
My hematocrit is high on TRT — what do I do?+
HCT above 54% requires intervention. Options: donate blood (therapeutic phlebotomy), reduce TRT dose, switch to more frequent lower-dose injections, or add a low-dose statin. Hydrate well before blood draws — dehydration falsely elevates HCT.
How do I interpret my results?+
Compare against the context-specific ranges, not just the lab's standard range. A Total T of 400 ng/dL is 'normal' by lab standards but may be suboptimal for TRT. The ranges shown here include community-validated optimal targets alongside standard references.
What if my lab uses different units?+
Use our Unit Converter tool to convert between US conventional units and SI/international units. The conversion factors are shown next to each biomarker.
Sources
- Endocrine Society Clinical Practice Guidelines — Testosterone Therapy (2018)
- UCSF Transgender Care — Guidelines for Gender-Affirming Hormone Therapy
- Fenway Health — Transgender Health Clinical Guidelines
- Quest Diagnostics / LabCorp — Standard Reference Ranges
- ADA — Standards of Medical Care in Diabetes (2025)
Doseline provides informational tools only. It is not a medical device and does not provide medical advice. Always consult a qualified healthcare provider.
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