Intranasal Peptide Calculator
Calculate sprays per dose, concentration, and bottle longevity for nasal spray peptides. Presets for BPC-157, Selank, Semax, and NAD+.
Quick Preset
Gut healing, cognitive support. Nasal route provides rapid systemic absorption.
Total peptide being dissolved
Total liquid in spray bottle
Most nasal spray bottles deliver 0.1 mL per pump. Check your bottle's specs.
Sprays per Dose
3 sprays per nostril is not needed — total across both nostrils
Concentration
100 mcg delivered per spray actuation
Doses per Bottle
50 total sprays in bottle
Bottle Longevity
At 2x daily dosing
Your target dose requires 2.5 sprays — you can't do a fractional spray, so rounding up to 3 gives you 300 mcg per dose. Adjust your liquid volume or peptide amount to get a whole number of sprays.
How Intranasal Peptide Delivery Works
The nasal mucosa is one of the most permeable membranes in the body. Blood vessels sit just beneath the thin epithelial layer, allowing peptides to absorb directly into systemic circulation — bypassing the digestive system entirely. This is why many peptides that would be destroyed by stomach acid work well as nasal sprays.
Bioavailability varies by peptide. Small peptides like Selank and Semax were specifically designed for intranasal delivery and achieve 60-90% bioavailability. Larger peptides like BPC-157 have lower nasal bioavailability (estimated 10-30%) but still reach therapeutic levels. NAD+ is too large for efficient nasal absorption on its own, so nasal NAD+ products often include absorption enhancers.
Preparing a Nasal Spray
Equipment Needed
- Nasal spray bottle (metered, amber glass or HDPE)
- Peptide vial (lyophilized powder)
- Bacteriostatic water or sterile saline
- Sterile syringe for reconstitution transfer
- Alcohol swabs for vial tops
Steps
- Clean the vial top with an alcohol swab
- Reconstitute the peptide with your chosen liquid volume
- Gently swirl (never shake) until fully dissolved
- Draw the solution into a sterile syringe
- Transfer into the nasal spray bottle
- Prime the spray mechanism (2-3 test pumps until mist appears)
- Store refrigerated (2-8C) and use within 30 days
Absorption and Storage
Maximising Absorption
- Clear your nose before spraying
- Tilt head slightly forward
- Aim toward the outer wall, not the septum
- Breathe gently through the nose after spraying
- Avoid blowing your nose for 10-15 minutes
- Alternate nostrils if doing multiple sprays
Storage
- Refrigerate at 2-8C after reconstitution
- Use amber glass bottles to protect from light
- Typical shelf life: 30 days reconstituted
- Never freeze reconstituted solution
- Keep the nozzle clean and capped
Spray Bottle Types
- 0.1 mL/spray — most common, standard metered dose
- 0.05 mL/spray — fine mist, better for high-concentration solutions
- 0.13 mL/spray — some pharmacy-grade bottles
- Always verify your bottle's delivery volume
Frequently Asked Questions
Is intranasal delivery as effective as injection?+
It depends on the peptide. Selank and Semax were designed for nasal delivery and are highly effective. BPC-157 has lower nasal bioavailability than subcutaneous injection, but many users report good results — the convenience trade-off is worth it for some protocols. Injection remains the gold standard for most peptides.
Can I use bacteriostatic water in a nasal spray?+
Yes, and many people do. The benzyl alcohol preservative (0.9%) helps prevent bacterial growth. Some prefer sterile saline (0.9% NaCl) for comfort, as it's isotonic with nasal mucosa and may cause less irritation. Both work — saline is gentler, BAC water lasts longer.
How do I know my spray bottle's volume per pump?+
Reputable suppliers specify this on the product listing (usually 0.1 mL/spray). If unknown, you can test: fill with a measured amount of water, count pumps until empty, and divide. Most standard nasal spray bottles deliver 0.1 mL per actuation.
Should I spray in one nostril or both?+
The total dose is across both nostrils — not per nostril. If your dose requires 2 sprays, do 1 in each nostril. This distributes the liquid across more surface area and improves absorption. For odd numbers, alternate which nostril gets the extra spray.
Why does my bottle seem to run out faster than calculated?+
Priming pumps (the first 2-3 sprays when you start a new bottle) use solution without delivering a full dose. There's also a small amount of dead volume that the pump can't reach at the bottom. Expect 5-10% less than the theoretical maximum.
Can I mix multiple peptides in one nasal spray?+
Some peptides are compatible in solution (like Selank + Semax), but not all combinations are stable together. Research compatibility before mixing. When in doubt, use separate bottles. The Blend Calculator on this site can help with ratio calculations.
What's the shelf life of a reconstituted nasal spray?+
Generally 28-30 days refrigerated. Some peptides degrade faster — BPC-157 is relatively stable, while more fragile peptides may only last 14-21 days. If the solution becomes cloudy or changes color, discard it.
Related Tools
Track your nasal spray protocols in the Doseline app — log each administration, get refill reminders, and see your dosing history across all delivery methods.
Doseline provides informational tools only. It is not a medical device and does not provide medical advice. Always consult a qualified healthcare provider.
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