HCG Reconstitution Calculator
Calculate exact draw volumes for reconstituted HCG. Enter your vial size, water volume, and target dose — get syringe units, concentration, and doses per vial.
Concentration after mixing: 2,500 IU/mL
Draw on U-100 syringe
0.20 mL volume
Volume per dose
Concentration
Full doses per vial
0.20 / 1 mL
Worked Example
Scenario: You have a 5,000 IU vial of HCG and add 2 mL of bacteriostatic water. You want to inject 250 IU per dose.
Concentration: 5,000 IU ÷ 2 mL = 2,500 IU/mL
Volume per dose: 250 IU ÷ 2,500 IU/mL = 0.10 mL
Syringe units: 0.10 mL × 100 = 10 units on a U-100 syringe
Doses per vial: 5,000 ÷ 250 = 20 full doses
Common HCG Protocols
| Use Case | Typical Dose | Frequency | Notes |
|---|---|---|---|
| TRT adjunct (fertility) | 250-500 IU | 2-3x/week | Maintains testicular function during TRT |
| PCT (post-cycle therapy) | 1,000-1,500 IU | EOD for 2-3 weeks | Stimulates endogenous testosterone production |
| Fertility treatment | 500-1,000 IU | 2-3x/week | Protocol varies — follow your clinician |
| HRT adjunct | 250-500 IU | 2x/week | Sometimes used alongside HRT protocols |
Storage Guidance
Before Reconstitution
Unreconstituted HCG can be stored at room temperature (up to 25C / 77F) until the expiration date on the vial. Some manufacturers recommend refrigeration for long-term storage. Always check the package insert.
After Reconstitution
Once mixed with bacteriostatic water, HCG must be refrigerated (2-8C / 36-46F). Most sources recommend using within 30 days, though some pharmacies state up to 60 days when stored properly. Never freeze reconstituted HCG. Discard if cloudy or discolored.
About HCG
Human Chorionic Gonadotropin (HCG) is a peptide hormone that mimics luteinizing hormone (LH). It stimulates the testes to produce testosterone and maintain fertility, which is why it is commonly used alongside TRT.
HCG is measured in International Units (IU) rather than milligrams, which makes reconstitution math different from most peptides. The concentration depends entirely on how much bacteriostatic water you add — more water means a lower concentration per mL, which means you draw more volume per dose.
There is no single "correct" amount of water to add. Using less water gives a more concentrated solution (smaller injection volume), while more water makes it easier to measure small doses accurately. A common approach is 2 mL of bacteriostatic water per 5,000 IU vial, yielding 2,500 IU/mL.
Frequently Asked Questions
How do I reconstitute HCG?+
Draw bacteriostatic water into a syringe, then slowly inject it into the HCG vial. Aim the stream at the glass wall — not directly onto the powder. Gently swirl (don't shake) until the powder fully dissolves. The solution should be clear and colorless.
Why does the amount of water matter?+
The amount of water determines the concentration. With 5,000 IU in 1 mL of water, each 0.1 mL = 500 IU. With 5,000 IU in 5 mL, each 0.1 mL = 100 IU. More water makes it easier to measure small doses accurately but requires drawing a larger volume.
What syringe should I use for HCG?+
A U-100 insulin syringe (either 0.5 mL or 1 mL) is standard. The 0.5 mL size has finer graduations, making it easier to measure small volumes. For very small doses, a 0.3 mL syringe provides the most precision.
How long does reconstituted HCG last?+
When mixed with bacteriostatic water and stored in the refrigerator (2-8C), reconstituted HCG typically lasts 30-60 days. If mixed with sterile water (no preservative), use within 48 hours. Always use bacteriostatic water for multi-dose vials.
Can I use regular sterile water instead of bacteriostatic water?+
For single-use, yes. For multi-dose vials (which you will puncture multiple times), always use bacteriostatic water. It contains 0.9% benzyl alcohol as a preservative that prevents bacterial growth after repeated needle punctures.
What does 'units' on an insulin syringe mean for HCG?+
On a U-100 insulin syringe, the markings (10, 20, 30, etc.) represent insulin units — but since the syringe is really just measuring volume, 10 units = 0.10 mL. You can use those markings to measure any liquid, including reconstituted HCG. The IU dose depends on your concentration.
Why is my HCG dosed in IU instead of mg?+
HCG potency is measured by biological activity (how strongly it stimulates LH receptors), not by weight. International Units (IU) standardize this across different manufacturers and batches. This is similar to how insulin and some other biologics are measured.
Related Tools
Track your HCG protocol in the Doseline app — log reconstitution dates, set injection reminders, and monitor your protocol alongside TRT or HRT tracking.
Doseline provides informational tools only. It is not a medical device and does not provide medical advice. Always consult a qualified healthcare provider.
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